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. 2012 Dec 26;59(2):174–179. doi: 10.1262/jrd.2012-148

Table 1. Comparison of mitochondrial DNA copy number of embryos 48 h after insemination among age groups.

Developmental stage mtDNA number (Mean ± SE)
All ages (n=49) Young (n=18) Middle (n=12) Aged (n=19) Aged (>180)* (n=7)
>7-cell 587,536 ± 104,277a 501,818 ± 163,051 674,356 ± 216,656 647,661 ± 203,372 118,408 ± 106,535
4-to 7-cell 463,796 ± 49,815ab 563,569 ± 52,664 357,376 ± 64,052 446,055 ± 81,851 301,181 ± 52,016
2-to 3-cell 397,852 ± 43,731ab 335,085 ± 74,154 336,218 ± 87,734 494,735 ± 69,936 240,436 ± 65,043
Non cleaved 353,039 ± 43,345b 444,072 ± 78,958 286,554 ± 69,663 332,980 ± 63,891 141,590 ± 64,694
All 418,020 ± 42,183ab 495,612 ± 91,018 342,556 ± 40,022 392,173 ± 60,445 216,472 ± 30,764

Embryos 48 h after insemination were divided into >7-cell stage, 4-to 7-cell stage and 2-to 3-cell stage embryos and noncleaved oocytes. mtDNA number per stage was measured. Cows were divided into age groups: Young 25–45 months, Middle, 45–120 months, Aged, >120 months, and Aged* >180 months. a–b: P<0.05.