Table 1. The effect insulin at 1.7 ρM on day 4, 5 and 6 blastocyst Oct4 and Nanog expression.

	TCNA	Oct4B	Oct4+NanogC
Day 4 control	39.4 ± 1.6*	36.4 ± 1.6	25.4 ± 1.4*
Day 4 insulin	43.6 ± 1.1*	39.5 ± 1.0	31.9 ± 1.2*
Day 5 control	89.1 ± 3.1‡	56.2 ± 2.3‡	33.7 ± 2.2‡
Day 5 insulin	110.5 ± 3.3‡	63.9 ± 2.3‡	48.2 ± 2.1‡
Day 6 control	153.4 ± 6.3	27.8 ± 2.9	4.8 ± 0.5†
Day 6 insulin	155.6 ± 5.2	29.1 ± 2.9	6.8 ± 0.6†

^A Total cell number of blastocysts on the day indicated. ^B Oct4 positive cell number of blastocysts on the day indicated. ^C Oct4 and Nanog positive cell number of blastocysts on the day indicated. Data is mean ± SEM. Like pairs a *, ^‡ and ^†, are significantly different within columns. N≥28 (3 replicate experiments).