Table II.
Internal or probe SNaPshot primers for analysis of nine SNP.
| Primer name | Sequence (5′-3′) | Size (bp) | Tm (°C) | ddNTP | Signal colour |
|---|---|---|---|---|---|
| WR | (A)15ATCATCCAGATGGGAAACT | 34 | 60.3 | C/T | Black/Red |
| DI | (A)19TGGGTGGTGAAGTCCACGC | 38 | 65.7 | C/T | Black/Red |
| CO | (A)23GGTGGGGAACAACCAGACGG | 43 | 66.7 | C/T | Black/Red |
| YT | (A)27ACTAGTTACCTGCAGGCCGT | 47 | 65.6 | G/T | Blue/Red |
| JS | (A)31AGTACTGCCTGGGGGCTGCC | 51 | 68.0 | C/T | Black/Red |
| KP | (A)35TGTCAATCTCCATCACTTCA | 55 | 63.5 | T/C | Red/Black |
| DO | (A)40TGACCTCAACTGCAACCAGT | 60 | 62,8 | T/C | Red/Black |
| JO | (A)45AATAGCCACAGCGTGTGTG | 64 | 63,1 | G/A | Blue/Green |
| HY | (A)53CTTAGCCTGGCTTAACCAAG | 73 | 63.9 | G/T | Blue/Red |