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. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: Curr Protoc Cell Biol. 2013 Dec 2;61:23.9.1–23.9.10. doi: 10.1002/0471143030.cb2309s61

Figure 2. 2-dimensional expansion of differentiation of ventricular myocytes.

Figure 2

Representative images of ventricular cells cultured in (a) DMSO, (b) BIO or (c) IWR stained for cardiac troponin T (cTnT) (green), Ki67 (red) and DAPI (blue). Scale bar represents 50μm. (d) Quantification of cTnT+ cells at day 1 (baseline) (E12.5+1), 3 (E12.5+4) and 6 (E12.5+7) additional days of culture in the presence or absence of BIO or IWR. Error bars indicate standard deviation. (n=3, each in 6 technical replicates for each time point).