Table 2.
Expression screening of DprE1 protein.
| Construct name | Fusion protein | MW DprE1 fusions (kDa) | Yield at 37°C (mg/l) | Yield at 17°C (mg/l) | |
|---|---|---|---|---|---|
| T5 promoter | |||||
| pT5-DprE1 | Only HisTag | 53.7 | 0.4 | 0.2 | |
| pT5-Sumo-DprE1 | Sumo | 65.5 | 12.3 | 14.1 | |
| pT5-Trx-DprE1 | Trx | 65.8 | 14.8 | 10.4 | |
| pT5-DsbC-DprE1 | DsbC | 77.4 | 6.2 | 4.7 | |
| pT5-MBP-DprE1 | MBP | 94.3 | 15.4 | 11.3 | |
| pT5-CelD-DprE1 | CelDnc | 114.8 | 19.5 | 12.8 | |
| T7 promoter | |||||
| pT7-DprE1 | Only HisTag | 53.7 | 0.1 | 0.2 | |
| pT7-Sumo-DprE1 | Sumo | 65.5 | 11.6 | 10.1 | |
| pT7-Trx-DprE1 | Trx | 65.8 | 12.4 | 9.8 | |
| pT7-DsbC-DprE1 | DsbC | 77.4 | 8.1 | 3.9 | |
| pT7-MBP-DprE1 | MBP | 94.3 | 12.8 | 15.8 | |
| pT7-CelD-DprE1 | CelDnc | 114.8 | 19.2 | ND |
After purification by IMAC, concentration of the entire fusions and yield was determined at 280 nm taking into account the different extinction coefficients. The expected molecular weight as well as construct name and characteristics are indicated.