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. 2014 Feb 25;5:67. doi: 10.3389/fpls.2014.00067

Table 1.

Control culture condition.

A. Modified K + ES seawater medium
Major nutrient (mM) NaNO3 2.35
K2HPO4 0.03
Iron (μM) Fe-Mn-EDTA  7.2
Thiamine HCl 200
Vitamin (μg/L) Biotin  1.5
B12  1.5
Trace minerals (μg/L) MnCl2.4H2O 178
ZnSO4. 7H2O  2.3
CoSO4. 7H2O  1.2
Na2MO4.H2O  7.2
CuSO4.5H2O  2.5
pH
TESa (pH = 8.0)
25 mM
B. Culture condition
Light quality Far-red lightb
Illumination time (h) Continuous (24 h)
Light intensity (μE) 20
Temperature (°C) 32
Salinity (‰) 33c
a

TES is 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid.

b

Single wavelength light emitting diodes (LEDs) 730 nm with 20 nm half width and viewing angle 30° (Nanning Lvxing Light Electronics Co., Ltd., China).

c

The sea salt concentration of 33.3 g in 1 L water is equivalent to 1 × natural seawater.