Table 1.
Control culture condition.
| A. Modified K + ES seawater medium | ||
|---|---|---|
| Major nutrient (mM) | NaNO3 | 2.35 |
| K2HPO4 | 0.03 | |
| Iron (μM) | Fe-Mn-EDTA | 7.2 |
| Thiamine HCl | 200 | |
| Vitamin (μg/L) | Biotin | 1.5 |
| B12 | 1.5 | |
| Trace minerals (μg/L) | MnCl2.4H2O | 178 |
| ZnSO4. 7H2O | 2.3 | |
| CoSO4. 7H2O | 1.2 | |
| Na2MO4.H2O | 7.2 | |
| CuSO4.5H2O | 2.5 | |
| pH |
TESa (pH = 8.0) |
25 mM |
| B. Culture condition | ||
| Light quality | Far-red lightb | |
| Illumination time (h) | Continuous (24 h) | |
| Light intensity (μE) | 20 | |
| Temperature (°C) | 32 | |
| Salinity (‰) | 33c | |
a
TES is 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid.
b
Single wavelength light emitting diodes (LEDs) 730 nm with 20 nm half width and viewing angle 30° (Nanning Lvxing Light Electronics Co., Ltd., China).
c
The sea salt concentration of 33.3 g in 1 L water is equivalent to 1 × natural seawater.