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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Stem Cell Res. 2013 Nov 5;12(1):233–240. doi: 10.1016/j.scr.2013.10.008

Figure 3. Foxd3 functions upstream of genes required for skeletal muscle development.

Figure 3

A. qRT-PCR data demonstrating the relative expression of Foxd3, Myf5, and Pax3 mRNA in Foxd3 induced mutant ESCs cultured for 24 (purple) and 48 (blue) hours. Red line indicates expression of these mRNAs in untreated ESCs. Error bars indicate SEM. *p< 0.05, ***p< 0.001 B. Model of Foxd3 and Sox15 in mESCs in the process of skeletal muscle differentiation. In a control progenitor cell (left), Foxd3 represses Sox15 allowing precise regulation of Pax3 and Myf5 and proper skeletal muscle development. In the absence of Foxd3 (right), Sox15 is upregulated, resulting in an increase in Pax3 and a decrease in Myf5 expression.