Table 1.

Target sequences of shRNA in 3D gene of FMDV used in this study.

Name	Target sequence (O/CHA/99, AF506822)	Oligonucleotide sequence
p3D1shRNA	5′-AAGACTCGCATTGTCGACGTC-3′   (nt 7196–7216 in the 3D region)	5′-GATCCGACTCGCATTGTCGACGTCTTCAAGAGAGACGTCGACAATGCGAGTCTTA-3′ (top strand) 5′-AGCTTAAGACTCGCATTGTCGACGTCTCTCTTGAAGACGTCGACAATGCGAGTCG-3′ (bottom strand)
p3D2shRNA	5′-AACAACATCTACGTGCTCTAC-3′   (nt 7598–7618 in the 3D region)	5′-GATCCCAACATCTACGTGCTCTACTTCAAGAGAGTAGAGCACGTAGATGTTGTTA-3′ (top strand) 5′-AGCTTAACAACATCTACGTGCTCTACTCTCTTGAAGTAGAGCACGTAGATGTTGG-3′′ (bottom strand)
p3D3shRNA	5′-AACATCTACGTGCTCTACGCT-3′   (nt 7601–7621 in the 3D region)	5′-GATCCCATCTACGTGCTCTACGCTTTCAAGAGA AGCGTAGAGCACGTAGATGTTA-3′ (top strand) 5′-AGCTTAACATCTACGTGCTCTACGCTTCTCTTGAAAGCGTAGAGCACGTAGATGG-3′′ (bottom strand)
p3D4shRNA	5′-AGACACTTCCACATGGACTA-3′   (nt 7828–7848 in the 3D region)	5′-GATCCGACACTTCCACATGGACTATTCAAGAGATAGTCCATGTGGAAGTGTCTTA-3′ (top strand) 5′-AGCTTAAGACACTTCCACATGGACTATCTCTTGAATAGTCCATGTGGAAGTGTCG-3′′ (bottom strand)
p3D5shRNA	5′-AAGTTACAGATCACTTTACCT-3′   (nt 8026–8046 in the 3D region)	5′-GATCCGTTACAGATCACTTTACCTTTCAAGAGA AGGTAAAGTGATCTGTAACTTA-3′ (top strand) 5′-AGCTTAAGTTACAGATCACTTTACCTTCTCTTGAAAGGTAAAGTGATCTGTAACG-3′′ (bottom strand)
pshRNA	5′-ACGGTGCCTTGCTCGCTAGAT-3′   (not homologous)	5′-GATCCGGTGCCTTGCTCGCTAGATTTCAAGAGAATCTAGCGAGCAAGGCACCGT A-3′ (top strand) 5′-AGCTTACGGTGCCTTGCTCGCTAGATTCTCTTGAAATCTAGCGAGCAAGGCACCG-3′ (bottom strand)

Sense and antisense sequence are underlined.