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Flatmount retina. One month following NaCl injection, the retina was removed and flatmounted onto a sylgard dish using cactus needles with the RGC layer facing up. The yellow boxes represent the areas of the retina where RGCs were counted (4 mm from the ONH), while the white boxes represent a distance of 2 mm from the ONH. The dotted lines represent retinal orientation maintained throughout the experiments. d, dorsal; n, nasal; t, temporal; v, ventral.
