Table 2.
Primary Antibodies.
| antibody | source | Catalog number | epitope | type |
|---|---|---|---|---|
| KCNQ1 | Santa Cruz Biotechnology | sc-10646 | C-terminal of human | Rabbit polyclonal |
| KCNQ4 | Neuromab | 75-082 | N-terminal of human | Mouse monoclonal |
| KCNQ5 | Chemicon | AB5599 | N-terminal of human KCNQ5 | Rabbit polyclonal |
| KCNE1 | Sigma-Aldrich | WH0003753M1 | aa 67–130 of human KCNE1 | Mouse monoclonal |
| KCNE1 | Alomone Labs | APC-008 | aa 67–129 of human KCNE1 | Rabbit polyclonal |
| KCNE1 | Santa Cruz Biotechnology | SC-16796 N-16 |
Internal region of human KCNE1 | Goat polyclonal |
| KCNE2 | Alomone Labs | APC-054 | aa 88–107 of rat KCNE2 | Rabbit polyclonal |
| KCNE3 | Alomone Labs | APC-118 | aa 81–93 of human KCNE3 | Rabbit polyclonal |
| KCNE4 | Gift from Dr. A.L. George, Jr in house | aa 73–94 of human KCNE4 | Rabbit polyclonal | |
| Kir7.1 | C-terminal of human Kir7.1 | Rabbit polyclonal | ||
| Na,K-ATPase | Abcam | AB7671 | full-length rabbit α1 subunit Na,K-ATPase | Mouse monoclonal |
| Red/Green Opsin | Millipore | AB5405 | human red/green opsin | Rabbit polyclonal |
| Blue Opsin | Millipore | AB5407 | human blue opsin | Rabbit polyclonal |
Secondary antibodies used include donkey anti-mouse IgG-HRP (cat. no. sc-2314), donkey anti-rabbit IgG-HRP (cat. no. sc-2313) from Santa Cruz Biotechnology (Santa Cruz, CA); Alexa Fluor488 goat anti-rabbit (H+L) and Alexa Fluor555 goat anti-mouse (H+L) (Invitrogen Corporation, Camarillo, CA).