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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Biochem Pharmacol. 2013 Nov 28;87(3):445–455. doi: 10.1016/j.bcp.2013.11.014

Fig. 7.

Fig. 7

Hsp70 is dispensable for autophagic flux in Hsp90 inhibitor-treated cells. RKO cells transfected with either a negative control (NEG) or Hsp70 siRNA (HSP70) were treated with geldanamycin or 17-AAG (250 nM) for 8 h. Bafilomycin A1 (400 nM) was added for the last 4 h of treatment where indicated. LC3 flux was calculated as the difference in densitometry values in the presence (+) and absence (−) of bafilomycin A1, after normalization to actin (loading control). Hsp70 immunoblots show inducible Hsp70 (Hsp70-1) as well as constitutive Hsp73 (Hsc70), which is not HSF1-dependent. Flux values are presented in bar graph, relative to vehicle-treated control (NEG) cells. Error bars represent standard deviations for n = 4 experiments and Western blots shows representative data, showing no statistically significant (p<0.05) differences between vehicle (DMSO)-treated and inhibitor-treated samples.