Fig. 2.

Effect of different cytokines on the proliferation of HK2 cells. Cells were treated for 48 h with different combinations of 1 ng/ml TGF-β1, 25 ng/ml TNF-α, 50 ng/ml IFN-γ, 0.5 μg/ml anti-Fas antibody (α-Fas), or vehicle (as control). At the end, an MTT assay was performed. Data represent the average ± SEM of 3 independent experiments performed in triplicate.