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. 2014 Feb 1;12(1):43–54. doi: 10.1089/adt.2013.520

Fig. 2.

Fig. 2.

Live cell imaging toxicity assessment. (A) iCell hepatocytes treated with indicated concentrations of amitryptiline for 72 h, then stained with Calcein AM, Hoechst 33258, and MitoTracker Orange. Images were taken using a 10× objective and analyzed using Multi-Wavelength Cell Scoring module. (B) Image processing by Multi-Wavelength Cell Scoring application module. Masks shown define Calcein AM staining (green), MitoTracker staining (orange), and Hoechst 33258 staining (grey). (C) Comparison of IC50s obtained by using total cells (nuclear count, blue circles), Calcein AM–positive cells (green triangles), or MitoTracker Orange–positive cells (red squares). NT, no significant toxicity detected from the four-parameter curve fit to the data.