Fig. 4.
Phenotypic characterization of toxicity by nuclear shape and intensity. (A) Images of iCell hepatocytes treated with 10 mM of staurosporine, stained with Hoechst 33258. Images were acquired using a 10× objective. (B) Concentration-dependent responses for several compounds using “average nuclear area” as a readout. The IC50s for the tested compounds were aflatoxin G1 (red squares), 1.9 μM; staurosporine (orange diamonds), 1.9 μM; aflatoxin B1 (blue circles), 5.5 μM; idarubicin (grey squares), 7.4 μM, mitomycin C (green triangles), 18 μM; and retinoic acid (purple circles), 482 μM.