Fig. 5.
Cytoskeleton integrity assessment by actin staining. (A) iCell hepatocytes treated with 30 mM of aflatoxin B1 for 72 h, then fixed and stained with AlexaFluor-488-Phalloidin and Hoechst 33258. (B) Concentration-dependent responses for several compounds using “number of actin-positive cells” as a readout. The IC50s for the tested compounds were aflatoxin G1 (red squares), 1.9 μM; staurosporine (orange diamonds), 2.0 μM; aflatoxin B1 (blue circles), 5.4 μM; idarubicin (grey squares), 7.4 μM; mitomycin C (green triangles), 19 μM; and retinoic acid (purple circles), 480 μM.