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. 2014 Feb 1;12(1):43–54. doi: 10.1089/adt.2013.520

Fig. 7.

Fig. 7.

(A) Autophagy detection assay. Image of iCell hepatocytes treated with rapamycin for 24 h and stained with Cyto-ID autophagy detection kit. Right: Analysis with Granularity module. (B) Phospholipidosis, lipid accumulation assay. Images of iCell hepatocytes treated with propranolol for 48 h. Phospholipidosis and steatosis detected using LipidTOX reagent showing phospholipids and neutral lipids as indicated. Concentration-dependent responses for phospholipidosis using “total granular area” output and corresponding IC50 values (in μM) for several compounds: amiodarone (blue circles), 18; cyclosporin A (orange diamonds), 1.3; propranolol (green triangles), 25.7; and chloroquine (red squares), 6.5.