Fig. 4.
mIA transcription factor (TF) analysis pipeline. (A) Schematic of the steps included in the TF pipeline. (B) Although many image cytometers apply proprietary image correction algorithms, source images (left) often contain uneven illumination artifacts observed in a profile plot (top right) examining pixel intensity (y-axis) versus position (x-axis) across the image that are eliminated by algorithms incorporated into mIA (bottom right). (C) Colored masks show primary (nuclei) and secondary (cells) object detection in HeLa cells. Each analysis produces a HTML report that includes (D) plate heat maps, (E) well response (y-axis) versus sample ID (x-axis), (F) replicate A (y-axis) versus replicate B (x-axis) response, and (G) waterfall Z-score plots that allows rapid visualization of plate results. (H) Dose–response plots and EC50 values are generated for each dose–response series indicated by well annotations.