Fig. 6.

mIA cell toxicity analysis pipeline and output. (A) Schematic of the steps included in the CT pipeline. (B) Toxicity index, a composite measurement, heat map (left) as well as heat maps for each measurement used in the index (right). (C) Summary bar chart indicating toxicity index (x-axis) for each sample type (y-axis) in each cell line (solid vs. open bars) indicated by well annotations. (D) K-Means Cluster analysis of defining toxicity patterns observed within dataset with an example detail available describing each cluster (inset). (E) HeLa cells were treated with 50 μM CCCP or DMSO for 7 h. A total of 120 wells were used for each treatment condition. Toxicity index calculated by examining cell count, nuclear size, nuclear shape, DNA content, and DNA pattern. CCCP, m-chlorophenyl hydrazone; DMSO, dimethyl sulfoxide.