Table 1.
Predefined Analysis Pipelines
| Type | Regions identified | Report content |
|---|---|---|
| Basic | Cell | Reports cell count and signal intensity for each image type in dataset per well. |
| Cytoplasm | ||
| Nucleus | ||
| Transcription factor | Cell | Reports cell count, four key metrics quantifying the transcription factor, and reporter accumulation if present. |
| Cytoplasm | ||
| Nucleus | ||
| Nuclear spot | Cell | Reports cell count, percent cells with spots, and metrics describing spot size and intensity. |
| Cytoplasm | ||
| Nucleus | ||
| Spots | ||
| Cell toxicity | Cell | Reports a calculated toxicity index based on markers present. Provides access to detailed toxicity and cell cycle reports. |
| Cytoplasm | ||
| Mitochondria | ||
| Nucleus | ||
| Pattern and intensity | Cell | Reports cell count, signal intensity, results clustered into those with distinct signal patterns. |
| Cytoplasm | ||
| Nucleus | ||
| Multinuclear | Cell | Identifies multinucleated cells based on nuclear position and reports cell count and basic signal intensity metrics. |
| Cytoplasm | ||
| Nucleus | ||
| Two-color correlation | Cell | Reports cell count, signal intensities, degree of pixel intensity correlation between two distinct signals. |
| Cytoplasm | ||
| Nucleus | ||
| Bacteria intensity | Bacteria | Reports bacteria count and multiple signal intensity metrics. |
| Yeast cell wall | Yeast | Reports yeast count, intensity metrics, and percent cells with septum. |
| Septum | ||
| Cell wall |