Fig. 2.

Quercetin improves insulin resistance and glucose uptake in HFD-induced obese mice. At 17 weeks old, blood glucose was measured in mice injected with 1 g/kg body weight D-glucose for glucose tolerance test (A) or 1.5 IU/kg body weight insulin for insulin tolerance test (B). (C) The mRNA level of GLUT4 in EAT. (D) The GLUT4 translocation in EAT. (E) Immunoblots for phospho-protein kinase B (AKT) (Ser 473) and AKT in EAT. GAPDH was used as reference gene in real-time PCR analysis. Quantification of GLUT4 and AKT was described as the ratio of PM GLUT4 to total GLUT4 and phosphorylated AKT to total AKT, respectively. Statistical difference between groups was shown using a nonparametric Mann-Whitney test; n = 8 per group in (A)–(E). * P < 0.05, ** P < 0.01, *** P < 0.001. All data in (A)–(E) are mean ± SEM.