Fig. 5.

Quercetin increases AMPKα1 activity and SIRT1 expression in EAT in HFD-fed mice. (A) Protein and phosphorylation levels of AMPKα1 in EAT. (B) Protein expression of SIRT1 in EAT. Quantification of AMPKα1 activity and SIRT1 expression was described as the ratio of phosphorylated AMPKα1 to total AMPKα1 and SIRT1 to β-actin, respectively. Statistical difference between groups was shown using a nonparametric Mann-Whitney test; n = 8 per group in (A) and (B). * P < 0.05, ** P < 0.01. All data in (A) and (B) are mean ± SEM.