Fig. 6.

Cholesterol depletion/repletion has no effect on the activation of NF-κB. Cholesterol depletion was performed on HuH-7 cells by incubation with 1.5% cyclodextrin (CD) for 1 h. Cholesterol repletion was performed by the addition of CD plus cholesterol (CD+Chol) for an additional hour. To assess the effects of rHDL on cholesterol mobilization, cells were treated with rHDL (rHDL) (final apoAI concentration 16 μmol/l or 0.45 mg/ml) for 16 h followed by the addition of CD plus cholesterol (rHDL+Chol) for an additional hour. Following incubations, nuclear proteins were extracted and NF-κB levels measured. Results are expressed as mean ± SEM (n = 3).