TABLE 2.
Confirmation of NF-κB-targeted genes in HuH-7 cells by RT-qPCR
| Gene Assignment | GenBank | Fold Change TNFα + HDL/TNFα | Function |
| A1AT, α-1 protease inhibitor | NM_001002236 | 0.78 ± 0.04a | Inhibits elastase, plasmin, thrombin, trypsin, and plasminogen activator |
| GAL3, galactose-specific lectin | NR_003225 | 0.64 ± 0.03b | Involvement in G protein-coupled receptor protein signaling pathway, cell differentiation, and extracellular matrix organization |
| GRO1, chemokine (C-X-C motif) ligand 1 | NM_001511 | 0.53 ± 0.04b | Chemokine, inflammatory response |
| GSTP1, glutathione S-transferase pi 1 | NM_000852 | 0.88 ± 0.04a | Important role in detoxification by conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles |
| IGFBP2, insulin-like growth factor binding protein 2 | NM_000597 | 0.69 ± 0.08a | Prolongs the half-life of IGFs, stimulates or inhibits the growth promoting effects of Igs on cell culture |
| IL8, interleukin 8 | NM_000584 | 0.73 ± 0.05a | Chemokine, major mediator of the inflammatory response |
| LAMB2, insulin-like growth factor binding protein 2 | NM_002292 | 0.74 ± 0.06b | Major noncollagenous constituent of basement membranes involved in cell adhesion |
| MAD3, max-associated protein 3 | NM_031300 | 0.90 ± 0.03b | Transcriptional regulator and part of the Myc superfamily. Binds with MAX and suppresses MYC-dependent cell transformation |
| MCP1, monocyte chemoattractant protein 1 | NM_002982 | 0.63 ± 0.08a | Chemokine, inflammatory response |
| MNSOD, manganese-containing superoxide dismutase | NM_001024465 | 0.65 ± 0.03b | Clears toxic oxidative radicals produced within cells |
| TP53, tumor suppressor p53 | NM_000546 | 0.89 ± 0.02b | Regulates target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. High levels are associated with transformation and malignancy |
| PRG1, p53-responsive gene 1 | NM_003897 | 0.65 ± 0.06b | |
| PTX3, pentraxin 3 | NM_002852 | 0.80 ± 0.04b | Role in regulation of inflammation |
| SAA1, serum amyloid A1 | NM_000331 | 0.71 ± 0.03b | Major acute inflammatory reactant, associated with HDL |
a
P < 0.05.
b
P < 0.01.