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. 2014 Mar;55(3):540–548. doi: 10.1194/jlr.M046342

Fig. 5.

Fig. 5.

A: mRNA expression of genes related to cholesterol, phospholipid, and bile acid metabolism and transport. Hepatic mRNA was extracted from mice treated with the indicated ASO for 4 weeks and fed a LD for the final 2 weeks. The mRNA expression of indicated genes was quantitated by real-time qPCR and the value is expressed as fold change related to C-ASO-treated WT mice, which was defined as 1. The bars represent the mean ± SE (n = 4 per group). B, C: Protein expression of cholesterol transporter Abcg8. Membrane protein (50 μg) from the above mice was separated by 8% SDS-PAGE. Abcg8 protein expression was determined by Western blot and quantitated by Kodak Image software, with β-actin as loading control. B: A representative image. C: A bar graph of relative Abcg8 abundance (mean ± SE of 4 mice per group) after normalizing expression to C-ASO-treated WT mice. *P < 0.05.