Table 5.
Expression of genes associated with inflammation, oxidative stress and metabolism in subcutaneous adipose from healthy cows (no uterine infection, NUI) or cows with subclinical endometritis (SCE). Data were generated via quantitative RT-PCR.
| GROUP
|
||||
|---|---|---|---|---|
| GENE | SCE | NUI | SEM | P = |
|
| ||||
| Transcription regulators | ||||
|
| ||||
| RXRA | −1.30 | −0.71 | 0.37 | 0.28 |
|
| ||||
| PPARG | 1.49 | 1.63 | 0.18 | 0.60 |
|
| ||||
| SREBF1 | 0.80 | 1.15 | 0.31 | 0.44 |
|
| ||||
| TP53 | 1.23(↑) | −0.20 | 0.44 | 0.04 |
|
| ||||
| NFIL3 | 0.68 | 0.44 | 0.12 | 0.20 |
|
| ||||
| Lipogenic enzymes | ||||
|
| ||||
| LPIN1 | −0.15 | 0.16 | 0.32 | 0.50 |
|
| ||||
| SCD | −1.21 | −0.12 | 0.80 | 0.36 |
|
| ||||
| FASN | 0.26 | 0.31 | 0.33 | 0.91 |
|
| ||||
| PLIN2 | −1.86(↓) | 0.20 | 0.57 | 0.02 |
|
| ||||
| Lipolytic-related (lipolysis) | ||||
|
| ||||
| ABHD5 | 0.09 | 0.08 | 0.26 | 0.98 |
|
| ||||
| LIPE | 0.14 | −0.10 | 0.19 | 0.38 |
|
| ||||
| PNPLA2 | 0.15 | 0.45 | 0.19 | 0.26 |
|
| ||||
| Insulin signaling | ||||
|
| ||||
| GHR | −0.09 | −0.08 | 0.30 | 0.98 |
|
| ||||
| SLC2A4 | 0.82(↓) | 1.44 | 0.21 | 0.03 |
|
| ||||
| IRS1 | −1.69(↓) | −1.02 | 0.13 | <0.01 |
|
| ||||
| Adipokines and hepatokines | ||||
|
| ||||
| ADIPOQ | 0.73 | 0.77 | 0.68 | 0.97 |
|
| ||||
| ANGPTL4 | −0.07 | −0.06 | 0.27 | 0.98 |
|
| ||||
| CCL5 | −1.23 | −1.26 | 0.52 | 0.98 |
|
| ||||
| BCL2 | −0.70 | −0.48 | 0.31 | 0.62 |
|
| ||||
| CCL2 | −1.34(↑) | −3.62 | 0.46 | <0.01 |
|
| ||||
| Oxidative stress | ||||
|
| ||||
| SOD2 | −2.44(↓) | 0.99 | 1.22 | 0.06 |
|
| ||||
| Cytokines/inflammatory mediators | ||||
|
| ||||
| IL6 | 0.36(↑) | −2.27 | 0.33 | <0.01 |
|
| ||||
| HP | −1.74 | −1.20 | 0.51 | 0.46 |
|
| ||||
| SAA3 | −2.64 | −0.68 | 1.26 | 0.29 |
|
| ||||
| NFKB1 | 1.26 | 0.98 | 0.28 | 0.98 |
|
| ||||
| STAT3 | 1.68 | 0.96 | 0.35 | 0.16 |