Table 4.

Summary of agonist EC₅₀ values for Leu-Met mutations at the position corresponding to GluN2A Leu812 in all other GluN1 and GluN2 subunits.

	Glutamate EC50, μM	Glycine EC50, μM
WT 2A	3.6 ± 0.26 (13)	1.1 ± 0.04 (7)
L808M/2A	0.39 ± 0.04 (10)*	0.12 ± 0.01 (10)*
WT 2B	1.3 ± 0.10 (12)	0.35 ± 0.01 (11)
2B-L813M	0.19 ± 0.01 (6)#	0.07 ± 0.007 (10)#
L808M/2B	0.16 ± 0.01 (10)#	0.06 ± 0.005 (11)#
WT 2C	0.82 ± 0.06 (10)	0.23 ± 0.2 (12)
2C-L808M	0.20 ± 0.01 (10)*	0.06 ± 0.01 (8)*
WT 2D	0.27 ± 0.01 (10)	0.10 ± 0.03 (16)
2D-L840M	0.05 ± 0.006 (14)*	0.016 ± 0.002 (13)*

The data were expressed as Mean ± SEM (n).

^*p < 0.05 compared to corresponding WT; unpaired t-test

^#p<0.05 compared to WT 2B; one way ANOVA, Tukey post hoc