Figure 5. Role of different EP receptors and cAMP on HIV-1 repression.

A) Infected PBL were treated with different agonists: butaprost (EP2) (10 µM), sulprostone (EP1/EP3) (10 µM) or misoprostol (EP4, EP3>EP2) (35 µM), or. Agp24 levels were quantified by ELISA 3 d later. B) PGE₂ interaction with EP2/EP4 receptors induces cAMP liberation. PBL were treated with PGE₂ (0.1 µM) for the indicated times, and intracellular cAMP was quantified as described under “Material and Methods”. C) Effect of dbcAMP (200 µM) on Agp24 production in human PBL after 3 days of infection. Statistical differences in comparison to HIV-1-infected (A,C) or control cells (B) *:p<0.05.; **:p<0.01.