Figure 6. PGE₂ regulates T cell actin polymerization throughout Epac/Rap1 and RhoA signaling.

A) PBL were infected and cultured in the presence of different concentrations of 8-CPT-2-OMe-cAMP (1, 10, 100 µM) or 6-Bnz-cAMP (1, 10, 100 µM) for 3 d. Supernatants were collected and Agp24 levels were determined by ELISA. B) Effect of PKA inhibitors (H89 5 µM, KT5720 200 nM) on PBL treated with PGE₂ 3d post-infection. C) Pull-down assay of active Rap on infected CEM-T cells for the indicated times. Western blots were probed for Rap1. D) Effect of PI3k and ERK1 inhibitors, wortmanin (100 nM) and PD98059 (10 µM) respectively, on HIV-1 replication in PGE₂-stimulated PBL. E) Immunoblot analysis of pull-down assay for Rho-GTP. Rho-A antibody was used to detect activated Rho-GTP from a pull-down assay using beads covalently bound to Rhotekin and also total Rho-A in cell lysates. Error bars indicate standard error values. Statistical differences in comparison to non-treated HIV-1-infected cells *:p<0.05.