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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Immunol. 2013 Dec 9;192(1):59–72. doi: 10.4049/jimmunol.1301513

Figure 3. Effect of AZD1480 on CD4+ T-cell Proliferation and Hematologic Parameters.

Figure 3

(A). C57BL/6 mice were injected i.p. with Vehicle Control (DMSO) or AZD1480 (25 mg/kg, 50 mg/kg or 100 mg/kg) for 4 days. Näive CD4+ T-cells isolated from the spleen of Vehicle Control or AZD1480 treated mice were labeled with carboxyfluorescein succinimidyl ester (CFSE), and stimulated with anti-CD3 (5 μg/ml) and anti-CD28 (2 μg/ml) Abs for 4 days. T-cell proliferation was analyzed by flow cytometry based on the dilution of CFSE intensity. (B). Peripheral blood from Vehicle Control or AZD1480 (25 mg/kg or 50 mg/kg) treated mice (n=3) was analyzed with HEMAVET®950. The number of red blood cells, hemoglobin, white blood cells, neutrophils, lymphocytes and monocytes were calculated.