Fig. 4.

A. Transient transfections of COS7 cells with varying quantity of either wild type or mutated lamin C-FP constructs. Cells were visualized by wide-field fluorescence microscopy with an excitation wavelength of 433 nm. The phenotype observed with mutated lamin C-FP is not due to a too elevated quantity of vectors used to transfect the cells. B. Representative Western blot analysis of COS7 cells transfected with the different lamin C-FP variants and using anti lamin A/C antibody. Results show equal over-expression for all construct.