Fig. 6.

A. COS7 cell nuclei transiently co-expressing wild type or mutated lamin A and lamin C constructs. Lamins A and C were inserted into pECFP-C1 and pDsRed2-C1 fluorescent expression vectors respectively. Cells were visualized by wide-field fluorescence microscopy with excitation wavelengths of 433 nm for lamin A-FP and 558 nm for DsRed2-lamin C. Compared to the wild type, the complex lamin A/C forms aggregates and the membrane appears granular and discontinued.

B. Laser-scanning confocal microscopy of COS7 cells nuclei transiently co-transfected with either wild type or mutated lamin A and lamin C. Lamin A (in red) and lamin C (in green) were inserted into pECFP-C1 and pEYFP-C1 fluorescent expression vectors; respectively. Excitation wavelengths were 433 nm for lamin A-FP and 558 nm for DsRed2-lamin C.