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. Author manuscript; available in PMC: 2014 Feb 25.

Published in final edited form as: Exp Cell Res. 2008 May 10;314(13):2362–2375. doi: 10.1016/j.yexcr.2008.04.017

Fig. 8 — A. FRAP experiments on wild type and L85R, D192G and/or R386K lamin C nuclear aggregates of COS7 cells. The boxed regions were bleached and the fluorescence recovery was monitored over a 210 s period. B. Quantitative experiments showing normalized fluorescence recovery after photobleaching of a targeted region of the lamin C nuclear aggregates in COS7 cells. (a) The fluorescence intensity in the bleached area is expressed as a relative recovery. Error bars indicates SEM, n=7. 1 is the level of fluorescence before bleaching. (b) We assessed the time after photobleaching required for fluorescence to recover the median value between the prebleach and just after the bleach (t_1/2) as a mean to reflect the dynamics of molecule.

Fig. 8 — A. FRAP experiments on wild type and L85R, D192G and/or R386K lamin C nuclear aggregates of COS7 cells. The boxed regions were bleached and the fluorescence recovery was monitored over a 210 s period. B. Quantitative experiments showing normalized fluorescence recovery after photobleaching of a targeted region of the lamin C nuclear aggregates in COS7 cells. (a) The fluorescence intensity in the bleached area is expressed as a relative recovery. Error bars indicates SEM, n=7. 1 is the level of fluorescence before bleaching. (b) We assessed the time after photobleaching required for fluorescence to recover the median value between the prebleach and just after the bleach (t_1/2) as a mean to reflect the dynamics of molecule.