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. Author manuscript; available in PMC: 2014 Feb 25.
Published in final edited form as: Basic Res Cardiol. 2010 Feb 3;105(3):365–377. doi: 10.1007/s00395-010-0085-4

Table 1.

Primer pairs and annealing temperatures used to amplify LMNA and TMPO coding regions

Primer sequences (5′ to 3′) PCR annealing temperature (°C)
LMNA Exon Forward Reverse
1A TCTGTCCTTCGACCCGAG GTAGACCGCCAAGCGATC 51
2A GGAGGACCTGCAGGAGCT GCCCTCTCACTCCCTTCC 50
3 CCTTCAAGTTCTTGTGTTCTGTGAC CCTAGCCCAGCCCAAGTCTGTC 58
4 GGCCTCCCAGGAACTAATTCTG CTCCCTGCCACCATCTGC 63
5 GCTGTAGCAGTGATGCCCAAC CCAAAGCCCTGAGAAGTGAAG 62
6 ATCCTGGAGAGAGTAGCCAG TCTAGTCAAGGCCAGTTGCC 60
7 CCCCACTTGGTCTCCCTCTCC CCCTGATGCAGCTGTATCCCC 60
8 GAGGCCTCAATTGCAGGCAGGC GAAAAGGACACTTACCCCAGC 62
9 GGAGCGCTGGGGTAAGTGTC CTCGTCCAGCAAGCAGCCAG 60
10 GTAAGCAGCAGGCCGGACAAAG GATGCCATGGAATATTCCTGTG 58
11A GGTCAGTCCCAGACTCGC ACCAGATTGTCCCCGAAG 55
11B GTCACTCGCAGCTACCGC CCACCTCGTCCTACCCCT 52
12 CTTGTCTGAGCCCCAGACTGGAG AGGGAAAAGGAAGGGAGGAGAAAT 65
TMPO 1 GTCTAAGGGGAAGGGTGGAG CAGACCCACACGTCAAAGAAC 60
2 CCAATTTGGTAGTGAGTTTGCA TAATTTGGGGTCCTGCTTCA 60
3 TGAGCTGCATCCTAAATGAAAC TGAGCTGCATCCTAAATGAAAC 57
4 GCTTTGTCTACCAGGGCAAATC GGCAGCCATCTTCACTCATC 60
5 AAACCAGGGTTCCCGATTA TGGATTAGTGTTGTCAGGAGGTT 60
6 CCAGTATGGCCGTTATTAAAGTA CTCCCTCCCACTCCAAAAA 60
7 AAGAGAGCCTTGGAAGCATGTG ACCATTGTACCTGGCTCCAAA 60
8 TCAGGGAATGTGCTTGGAAT GCAGTTTTTATTCAGCAGAGAA 60