Table 1.

Primer pairs and annealing temperatures used to amplify LMNA and TMPO coding regions

		Primer sequences (5′ to 3′)		PCR annealing temperature (°C)
LMNA	Exon	Forward	Reverse
	1A	TCTGTCCTTCGACCCGAG	GTAGACCGCCAAGCGATC	51
	2A	GGAGGACCTGCAGGAGCT	GCCCTCTCACTCCCTTCC	50
	3	CCTTCAAGTTCTTGTGTTCTGTGAC	CCTAGCCCAGCCCAAGTCTGTC	58
	4	GGCCTCCCAGGAACTAATTCTG	CTCCCTGCCACCATCTGC	63
	5	GCTGTAGCAGTGATGCCCAAC	CCAAAGCCCTGAGAAGTGAAG	62
	6	ATCCTGGAGAGAGTAGCCAG	TCTAGTCAAGGCCAGTTGCC	60
	7	CCCCACTTGGTCTCCCTCTCC	CCCTGATGCAGCTGTATCCCC	60
	8	GAGGCCTCAATTGCAGGCAGGC	GAAAAGGACACTTACCCCAGC	62
	9	GGAGCGCTGGGGTAAGTGTC	CTCGTCCAGCAAGCAGCCAG	60
	10	GTAAGCAGCAGGCCGGACAAAG	GATGCCATGGAATATTCCTGTG	58
	11A	GGTCAGTCCCAGACTCGC	ACCAGATTGTCCCCGAAG	55
	11B	GTCACTCGCAGCTACCGC	CCACCTCGTCCTACCCCT	52
	12	CTTGTCTGAGCCCCAGACTGGAG	AGGGAAAAGGAAGGGAGGAGAAAT	65
TMPO	1	GTCTAAGGGGAAGGGTGGAG	CAGACCCACACGTCAAAGAAC	60
	2	CCAATTTGGTAGTGAGTTTGCA	TAATTTGGGGTCCTGCTTCA	60
	3	TGAGCTGCATCCTAAATGAAAC	TGAGCTGCATCCTAAATGAAAC	57
	4	GCTTTGTCTACCAGGGCAAATC	GGCAGCCATCTTCACTCATC	60
	5	AAACCAGGGTTCCCGATTA	TGGATTAGTGTTGTCAGGAGGTT	60
	6	CCAGTATGGCCGTTATTAAAGTA	CTCCCTCCCACTCCAAAAA	60
	7	AAGAGAGCCTTGGAAGCATGTG	ACCATTGTACCTGGCTCCAAA	60
	8	TCAGGGAATGTGCTTGGAAT	GCAGTTTTTATTCAGCAGAGAA	60