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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Jan;1840(1):656–666. doi: 10.1016/j.bbagen.2013.10.028

Figure 8.

Figure 8

13C1 PA in MbCO, deoxy Mb, and Tris exhibit contrasting spectra: A) 0.8mM MbCO in 30mM Tris buffer at pH 7.4 at 35°C B) 0.8 mM MbCO with 0.8 mM PA. PA peaks appear at 172 and 182 ppm, fig 8A–8B C) 0.8mM deoxy Mb D) 0.8mM deoxy Mb with 0.8 mM PA E) 3.2 mM PA in Tris buffer, pH 7.4. 13C1 PA appears at 172 ppm. F) 3.2 mM PA in Tris buffer, pH 9.5. 13C1 PA appears at 184 ppm.