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. 2014 Feb 25;9(2):e88951. doi: 10.1371/journal.pone.0088951

Figure 6. Effect of SA or COR pretreatment on flg22-induced MYB51 transcript accumulation and callose deposition of Arabidopsis seedlings.

Figure 6

(A) MYB51 transcripts were measured in 8-day-old seedlings 1 h after treatment with 1 µM flg22. Data represent SD. All quantitative gene expression measurements were performed using technical triplicates and biological duplicates. (B–C) Eight-day-old seedlings were pre-incubated with SA (100 µM) or COR (0.5 µM) for 24 h, after which the seedlings treated with flg22 for 1 h were stained with aniline blue. Relative callose intensities were quantified as the number of fluorescent callose-corresponding pixels relative to the total number of pixels covering plant material. Values represent SE, n>6. Differential letter types indicated significant differences (α = 0.05) by one-way ANOVA and Tukey HSD test of comparisons between plant genotypes with individual treatment.