Table 4. Effect of PaCDase on levels of expression of sphingosine kinase and S1P receptors genes.

Name	-	PaCDase	ratio
SPHK1	2.39	6.40	2.68
SPHK2	0.98	1.07	1.09
S1P(1)/EDG1	0.28	0.28	0.97
S1P(2)/EDG5	0.57	0.42	0.73
S1P(3)/EDG3	0.38	1.00	2.70
S1P(4)/EDG6	0.30	0.40	1.22
S1P(5)/EDG8	0.43	0.23	0.55

Nitrocellulose filters with 1 mU/ml PaCDase or 0.1% DMSO (-) in Tris-buffered saline containing 0.1% Triton X-100 were placed onto the stratum corneum. The cells were incubated for 24 h, and DNA microarray analysis was performed. The results shown are representative of 3 experiments.