Table 4. Key differentially expressed genes following drought challenge in P. grandis and U. tetralasmus.
| Gene description | NCBI Accession number | P. grandis | U. tetralasmus | t-test p<0.05 | ||||||
| Contig ID | FC | p-value | Contig ID | FC | p-value | |||||
| Chaperone/HSP ( p = 0.0277) | ||||||||||
| 60 kDa heat shock protein | EKC31862.1 | fl_ctg_778 | 2.7 | 4.7E-05 | ph_ctg_636 | 2.0 | 7.1E-03 | |||
| 78 kDa glucose-regulated protein | EKC33663.1 | fl_ctg_1364 | 6.0 | 3.6E-43 | ph_ctg_1443 | 3.8 | 2.6E-31 | |||
| Alpha-crystallin B chain | EKC27067.1 | fl_ctg_1125 | 35.8 | 2.8E-11 | ph_ctg_1374 | 7.2 | 1.5E-152 | * | ||
| DnaJ-like protein subfamily A member 1 | EKC32228.1 | fl_ctg_129 | 3.4 | 1.3E-02 | ph_ctg_1424 | 2.2 | 1.9E-04 | |||
| DnaJ-like protein subfamily A member 2 | EKC18925.1 | fl_ctg_188 | 2.0 | 2.5E-15 | ph_ctg_58 | 1.6 | 2.2E-05 | * | ||
| Endoplasmin | EKC38233.1 | fl_ctg_1292 | 3.1 | 7.1E-35 | ph_ctg_790 | 2.1 | 1.8E-08 | * | ||
| Heat shock 10kDa protein | NP_001084708.1 | fl_ctg_489 | 5.3 | 4.9E-03 | ph_ctg_1163 | 2.1 | 4.7E-06 | * | ||
| Heat shock 70 kDa protein 12A, type 1 | EKC29170.1 | fl_ctg_1046 | −2.7 | 1.5E-02 | ph_ctg_1795 | −5.8 | 4.2E-04 | |||
| Heat shock 70 kDa protein 14 | EKC26064.1 | fl_ctg_506 | 4.6 | 4.5E-05 | nde_ctg_5 | 1.3 | 4.5E-01 | * | ||
| Heat shock protein 68 | EKC22243.1 | fl_ctg_2557 | 24.6 | 1.7E-02 | ph_ctg_1676 | 15.9 | 1.2E-06 | |||
| Heat shock protein 70 B2, type2 | EKC30019.1 | fl_ctg_1763 | 4.61 | 0 | ph_ctg_1662 | 1.66 | 4.3E-21 | * | ||
| Heat shock protein beta-1, type 2 | EKC40046.1 | fl_ctg_683 | 21.5 | 1.6E-10 | ph_ctg_2231 | 10.6 | 1.8E-243 | * | ||
| Heat shock protein beta-1, type 3 | EKC40046.1 | fl_ctg_1192 | 58.7 | 5.0E-07 | ph_ctg_1086 | 21.3 | 9.3E-78 | * | ||
| Heat shock protein HSP 90-alpha 1 | EKC25687.1 | fl_ctg_2540 | 9.5 | 5.5E-18 | ph_ctg_1044 | 9.5 | 8.9E-65 | |||
| Stress-70 protein, mitochondrial | EKC18038.1 | fl_ctg_1597 | 3.4 | 7.0E-03 | ph_ctg_1927 | 1.6 | 3.8E-08 | |||
| BAG molecular chaperone regulator 4 | EKC42633.1 | fl_ctg_2336 | 27.9 | 6.0E-16 | ||||||
| Calnexin | EKC32723.1 | fl_ctg_224 | 6.1 | 2.4E-08 | ||||||
| Calreticulin | EKC23905.1 | fl_ctg_1087 | 2.7 | 1.1E-16 | ||||||
| DnaJ-like protein subfamily B member 4 | EKC35183.1 | fl_ctg_2051 | 6.3 | 1.1E-41 | ||||||
| Heat shock 70 kDa protein 12A, type 2 | EKC19496.1 | fl_ctg_2338 | 3.6 | 2.7E-02 | ||||||
| Heat shock factor protein 1 | EKC33988.1 | fl_ctg_757 | 6.1 | 2.3E-02 | ||||||
| Heat shock protein 70 B2, type1 | EKC21713.1 | fl_ctg_2493 | 8.9 | 2.6E-03 | ||||||
| Heat shock protein beta-1, type 1 | EKC27576.1 | fl_ctg_1863 | 173.2 | 2.9E-10 | ||||||
| HSPB1-associated protein 1 | EKC31176.1 | fl_ctg_1565 | 4.0 | 1.3E-05 | ||||||
| Heat shock 70 kDa protein 12B | EKC29173.1 | ph_ctg_1045 | 7.4 | 3.2E-06 | ||||||
| Antioxidant/Oxidative Stress Response (p = 0.1534) | ||||||||||
| Calpain-5 | EKC27521.1 | fl_ctg_1914 | 1.7 | 1.1E-02 | ph_ctg_688 | 4.4 | 1.1E-05 | |||
| Ceruloplasmin | EKC34134.1 | fl_ctg_810 | 4.8 | 6.8E-31 | ph_ctg_1266 | −17.3 | 1.6E-02 | * | ||
| Dual oxidase | EKC42615.1 | fl_ctg_941 | −7.0 | 2.1E-03 | ph_ctg_2002 | −4.4 | 2.6E-02 | |||
| Glutathione peroxidase 1 | EKC25678.1 | fl_ctg_2179 | 2.9 | 2.6E-14 | ph_ctg_1277 | 2.2 | 9.9E-12 | |||
| Pantetheinase | EKC31982.1 | fl_ctg_1534 | 2.6 | 1.0E-04 | nde_ctg_11 | 1.2 | 6.5E-01 | |||
| Protein toll | EKC33894.1 | fl_ctg_829 | 10.9 | 2.7E-04 | ph_ctg_1155 | 3.1 | 4.9E-02 | * | ||
| Selenide, water dikinase | EKC33185.1 | nde_ctg_4 | 1.3 | 2.4E-02 | ph_ctg_1540 | 2.3 | 7.7E-20 | * | ||
| Selenoprotein M | NP_001186811.1 | fl_ctg_1703 | −3.7 | 3.3E-02 | ph_ctg_1989 | −2.4 | 4.2E-04 | |||
| Selenoprotein P, plasma, 1b | AAH86844.1 | fl_ctg_2541 | −2.1 | 1.3E-04 | ph_ctg_1412 | −2.1 | 0 | |||
| Selenoprotein T | XP_974477.1 | fl_ctg_718 | 3.0 | 6.9E-10 | ph_ctg_1960 | 2.0 | 3.6E-04 | |||
| Superoxide dismutase | EKC26875.1 | fl_ctg_1390 | −3.2 | 1.4E-04 | ph_ctg_460 | −3.4 | 2.2E-02 | |||
| Thioredoxin reductase 3 | EKC27867.1 | fl_ctg_1736 | −1.8 | 2.1E-02 | ph_ctg_2174 | −2.0 | 7.8E-06 | |||
| X-box-binding protein 1 | EKC27673.1 | fl_ctg_2520 | 33.0 | 2.0E-67 | ph_ctg_388 | 2.5 | 1.1E-06 | * | ||
| Elongation factor 1-alpha | EKC33063.1 | fl_ctg_1966 | 2.5 | 1.4E-02 | ||||||
| Ubiquitin-protein ligase E3C | EKC38417.1 | fl_ctg_1472 | 9.8 | 1.5E-03 | ||||||
| Calcium homeostasis endoplasmic reticulum | EKC18572.1 | ph_ctg_2138 | 6.1 | 5.9E-03 | ||||||
| Selenoprotein W | NP_001159799.1 | ph_ctg_685 | −1.7 | 3.6E-07 | ||||||
| Cell proliferation/Apoptosis (p = 0.2148) | ||||||||||
| AP-2 complex subunit mu-1 | EKC19414.1 | fl_ctg_415 | −2.0 | 2.3E-02 | ph_ctg_784 | 21.5 | 1.6E-05 | * | ||
| Apoptosis regulator R1 | EKC20695.1 | fl_ctg_2184 | 1.7 | 6.0E-03 | ph_ctg_159 | 2.1 | 1.7E-02 | |||
| BNIP1 | EKC19480.1 | fl_ctg_43 | 2.8 | 4.1E-02 | ph_ctg_164 | 11.0 | 1.3E-13 | * | ||
| BNIP3 | EKC35529.1 | fl_ctg_593 | 3.8 | 1.6E-07 | ph_ctg_2228 | 3.2 | 2.3E-13 | |||
| Bcl-2-like protein 1 | EKC30554.1 | fl_ctg_1908 | 2.8 | 1.7E-27 | ph_ctg_493 | 3.4 | 5.4E-06 | |||
| Cathepsin L-like cysteine proteinase | EKC36430.1 | fl_ctg_2106 | 2.3 | 3.6E-08 | ph_ctg_523 | −2.1 | 5.0E-02 | * | ||
| Cold shock domain-containing protein E1 | EKC28502.1 | fl_ctg_1124 | 1.8 | 2.3E-10 | ph_ctg_2058 | 2.0 | 2.6E-04 | |||
| Corticosteroid 11-beta-dehydrogenase 2 | EKC40090.1 | fl_ctg_1159 | 32.2 | 1.2E-06 | ph_ctg_608 | 7.4 | 5.0E-02 | * | ||
| Cyclin-I | EKC18711.1 | fl_ctg_2271 | 2.0 | 6.2E-09 | ph_ctg_2184 | 2.0 | 1.7E-05 | |||
| E3 ubiquitin-protein ligase MIB2 | EKC39104.1 | fl_ctg_2263 | −6.2 | 2.1E-02 | ph_ctg_1106 | 3.6 | 4.6E-05 | * | ||
| GADD45 alpha | EKC31645.1 | fl_ctg_62 | 28.2 | 1.2E-05 | nde_ctg_12 | −1.2 | 6.3E-01 | * | ||
| Inhibitor of apoptosis protein | EKC20774.1 | fl_ctg_1444 | 14.7 | 7.8E-24 | ph_ctg_1060 | 11.9 | 6.7E-03 | |||
| Krueppel-like factor 5 | EKC17625.1 | fl_ctg_1639 | 25.8 | 8.1E-60 | ph_ctg_1332 | 3.7 | 3.0E-04 | * | ||
| Polycomb complex protein BMI-1 | EKC23428.1 | fl_ctg_1135 | 2.7 | 7.7E-06 | ph_ctg_1337 | 2.9 | 2.0E-08 | |||
| Protein BTG1 | EKC25710.1 | fl_ctg_1732 | 9.1 | 8.4E-175 | ph_ctg_167 | 1.5 | 1.0E-02 | * | ||
| Serine/threonine-protein kinase Pim-3 | EKC19760.1 | fl_ctg_1493 | 3.7 | 6.6E-13 | ph_ctg_1242 | −2.4 | 1.3E-08 | * | ||
| Stress-induced-phosphoprotein 1 | EKC18743.1 | fl_ctg_1646 | 3.5 | 3.5E-08 | ph_ctg_1651 | 2.5 | 9.8E-08 | * | ||
| TMBIM4 | EKC24718.1 | fl_ctg_853 | 2.8 | 1.3E-02 | ph_ctg_2481 | 2.0 | 3.1E-12 | * | ||
| Ubiquitin | EKC42613.1 | fl_ctg_971 | 14.0 | 5.0E-13 | ph_ctg_231 | 2.0 | 1.0E-31 | * | ||
| Antigen KI-67 | EKC25595.1 | fl_ctg_1316 | −4.9 | 1.3E-02 | ||||||
| Caspase-10 | EKC25819.1 | fl_ctg_1166 | 8.2 | 2.8E-05 | ||||||
| CDH1-D | AAL31950.1 | fl_ctg_1990 | 2.4 | 2.8E-06 | ||||||
| MAP kinase signal-integrating kinase 1 | EKC18571.1 | fl_ctg_1707 | 3.9 | 1.8E-10 | ||||||
| Protein SET | EKC28777.1 | ph_ctg_761 | −2.6 | 2.7E-04 | ||||||
| Putative inhibitor of apoptosis | EKC26950.1 | ph_ctg_868 | 86.8 | 6.5E-26 | ||||||
| Ubiquitin-conjugating E2 variant 1 (UEV1A) | EKC31486.1 | ph_ctg_910 | 4.5 | 5.1E-14 | ||||||
| Energy Metabolism (p = 0.1482) | ||||||||||
| 5′-AMP-activated protein kinase beta-2 | EKC39833.1 | fl_ctg_2083 | −4.3 | 1.2E-05 | ph_ctg_2306 | −2.1 | 2.9E-02 | * | ||
| ATP synthase F0 subunit 6 | YP_003355006.1 | fl_ctg_2323 | −7.1 | 5.8E-08 | nde_ctg_6 | −3.3 | 2.7E-01 | |||
| Cholecystokinin receptor | EKC30410.1 | fl_ctg_1030 | 3.8 | 1.1E-04 | ph_ctg_337 | −3.3 | 4.9E-02 | * | ||
| Cytochrome b | YP_003354999.1 | fl_ctg_1821 | −5.6 | 0 | ph_ctg_589 | −2.7 | 2.1E-02 | |||
| Cytochrome c oxidase subunit I | YP_003354995.1 | fl_ctg_1261 | −3.4 | 0 | ph_ctg_288 | −1.6 | 1.3E-13 | * | ||
| Cytochrome c oxidase subunit II | YP_003354996.1 | fl_ctg_1138 | −3.3 | 0 | ph_ctg_23 | −1.9 | 3.1E-04 | |||
| Cytochrome c oxidase subunit III | YP_003355007.1 | fl_ctg_1487 | −4.5 | 0 | nde_ctg_7 | −2.4 | 3.1E-01 | |||
| Glutamine synthetase 2 cytoplasmic | EKC27195.1 | fl_ctg_1817 | 5.3 | 1.5E-24 | ph_ctg_1688 | 2.0 | 1.6E-24 | * | ||
| Group XIIA secretory phospholipase A2 | EKC23468.1 | fl_ctg_2457 | 2.8 | 1.6E-03 | ph_ctg_1198 | 10.9 | 5.0E-05 | * | ||
| NADH dehydrogenase subunit 1 | YP_003355001.1 | fl_ctg_1537 | −6.0 | 0 | nde_ctg_14 | −1.3 | 8.8E-01 | * | ||
| NADH dehydrogenase subunit 4 | YP_003355003.1 | fl_ctg_1864 | −2.0 | 1.5E-03 | nde_ctg_8 | −1.1 | 9.5E-01 | |||
| NADH dehydrogenase subunit 5 | YP_003355000.1 | fl_ctg_1887 | −3.8 | 2.5E-12 | ph_ctg_503 | 1.7 | 1.5E-03 | * | ||
| Succinyl-CoA ligase | EKC28914.1 | fl_ctg_1283 | −2.2 | 2.7E-03 | ph_ctg_1091 | −3.5 | 6.2E-06 | |||
| ATF5 activating transcription factor 5 | EKC37224.1 | fl_ctg_360 | 2.4 | 9.5E-07 | ||||||
| Immune (p = 0.7569) | ||||||||||
| B-cell lymphoma 3-encoded protein | EKC39819.1 | fl_ctg_200 | 6.2 | 1.2E-02 | ph_ctg_1522 | −2.4 | 4.0E-06 | * | ||
| Histone H4 transcription factor | EKC36777.1 | fl_ctg_2257 | 6.0 | 5.8E-08 | nde_ctg_9 | 1.9 | 5.5E-01 | * | ||
| Interferon regulatory factor 2 | EKC43156.1 | fl_ctg_1121 | −2.5 | 1.4E-06 | ph_ctg_76 | −4.2 | 3.3E-10 | |||
| Interleukin-1 receptor-associated kinase 1BP1 | AER35157.1 | nde_ctg_2 | 1.1 | 6.0E-01 | ph_ctg_1648 | −1.7 | 1.3E-02 | * | ||
| Lipopolysaccharide-binding protein | EKC33909.1 | fl_ctg_2167 | −2.8 | 9.7E-03 | ph_ctg_1475 | −2.0 | 1.2E-03 | |||
| LPS-induced tumor necrosis factor-alpha | EKC36231.1 | fl_ctg_1784 | −4.3 | 2.0E-03 | ph_ctg_1823 | −2.3 | 4.9E-02 | |||
| Lysozyme | EKC39289.1 | fl_ctg_477 | 2.1 | 4.1E-02 | ph_ctg_2014 | 2.3 | 3.0E-10 | |||
| NLR family, pyrin domain containing 3 | XP_003730107.1 | fl_ctg_758 | 4.5 | 9.8E-04 | ph_ctg_1933 | 2.7 | 1.5E-03 | |||
| NF-kappa-B inhibitor cactus | EKC37718.1 | fl_ctg_2206 | 3.9 | 1.9E-18 | ph_ctg_935 | 2.1 | 2.5E-04 | * | ||
| Peptidoglycan-recognition protein SC2 | EKC42861.1 | fl_ctg_1392 | 3.4 | 3.0E-03 | ph_ctg_1182 | 3.4 | 1.6E-13 | |||
| Phospholipid scramblase 2, partial | EKC25020.1 | fl_ctg_585 | −4.0 | 2.0E-02 | ph_ctg_894 | 14.3 | 5.4E-06 | * | ||
| Serine/threonine-protein kinase TBK1 | EKC41453.1 | fl_ctg_1373 | 7.0 | 3.2E-31 | ph_ctg_1115 | 11.3 | 3.4E-03 | |||
| Slit-like protein 1 protein | EKC33230.1 | fl_ctg_1877 | 6.5 | 7.0E-06 | nde_ctg_13 | 1.2 | 5.2E-01 | * | ||
| TNFAIP3-interacting protein 2 | EKC38434.1 | fl_ctg_840 | 4.7 | 4.3E-04 | ph_ctg_1846 | 2.5 | 2.2E-02 | |||
| TNF receptor-associated factor 2 | EKC31562.1 | nde_ctg_1 | 1.9 | 5.4E-01 | ph_ctg_783 | 4.5 | 1.7E-03 | * | ||
| Toll-like receptor 2 type-2 | EKC18337.1 | fl_ctg_1332 | 3.8 | 9.9E-06 | ph_ctg_1237 | 4.0 | 1.3E-10 | |||
| Toll-like receptor 3 | EKC35956.1 | fl_ctg_1369 | −3.0 | 3.4E-03 | ph_ctg_26 | 4.6 | 7.1E-03 | * | ||
| 14-3-3 protein epsilon | EKC29146.1 | fl_ctg_1686 | −4.2 | 9.8E-13 | ||||||
| Myeloid differentiation primary response 88 | EKC40065.1 | fl_ctg_2378 | 3.6 | 3.0E-03 | ||||||
| Tax1-binding protein 1-like protein B | EKC20118.1 | fl_ctg_2123 | −1.5 | 8.1E-03 | ||||||
| 14-3-3 protein zeta | EKC18419.1 | ph_ctg_1953 | −7.8 | 1.3E-04 | ||||||
| Complement C3 | EKC24393.1 | ph_ctg_2299 | −1.6 | 1.7E-06 | ||||||
| Defensin | ADO32580.1 | ph_ctg_2420 | 100.1 | 1.9E-06 | ||||||
| EIF4EBP1 | EKC38736.1 | ph_ctg_889 | 2.5 | 1.1E-07 | ||||||
| NLR family, pyrin domain containing 1 | XP_002731351.1 | ph_ctg_1301 | 13.2 | 2.2E-03 | ||||||
| TNF receptor-associated factor 3 | EKC22057.1 | ph_ctg_2244 | 3.3 | 4.5E-03 | ||||||
| Toll-like receptor 13 | EKC26527.1 | ph_ctg_1671 | −10.7 | 1.8E-02 | ||||||
| Cytoskeletal (p = 0.0149) | ||||||||||
| Collagen alpha-2(I) chain | EKC19703.1 | fl_ctg_1852 | −2.3 | 1.0E-02 | ph_ctg_1792 | −1.9 | 1.5E-05 | |||
| Fibrillin-1-like | EKC40135.1 | fl_ctg_1999 | −2.3 | 2.0E-02 | ph_ctg_2292 | −6.5 | 1.2E-03 | * | ||
| Microtubule-associated protein 2 | EKC35104.1 | fl_ctg_1783 | 40.1 | 4.4E-08 | ph_ctg_664 | 5.9 | 6.1E-04 | * | ||
| Myosin catalytic light chain LC-1 | EKC20656.1 | nde_ctg_3 | 1.2 | 8.4E-02 | ph_ctg_1040 | −13.0 | 2.1E-04 | * | ||
| Protocadherin Fat 4 | EKC27752.1 | fl_ctg_2135 | 1.9 | 8.5E-03 | ph_ctg_1385 | −16.5 | 2.8E-07 | * | ||
| Ras-like GTP-binding protein Rho1 | EKC37733.1 | fl_ctg_982 | 15.5 | 3.8E-03 | ph_ctg_997 | −2.7 | 4.9E-02 | * | ||
| Tubulin alpha-1C chain | EKC19808.1 | fl_ctg_738 | −2.4 | 1.3E-10 | ph_ctg_1023 | −2.2 | 5.4E-09 | |||
| Tubulin beta chain | EKC30036.1 | fl_ctg_408 | 33.1 | 7.9E-31 | ph_ctg_2439 | 3.6 | 1.2E-03 | * | ||
| Dynein heavy chain 6, axonemal | EKC31674.1 | fl_ctg_2151 | −2.8 | 7.6E-04 | ||||||
| Tubulin alpha chain | EKC22339.1 | fl_ctg_442 | −3.4 | 3.7E-08 | ||||||
| Other (p = 0.8545) | ||||||||||
| Chitin synthase 1 | EKC23809.1 | fl_ctg_835 | −3.5 | 2.1E-04 | ph_ctg_1713 | −4.3 | 3.5E-06 | |||
| Chloride intracellular channel exc-4 | EKC30516.1 | fl_ctg_1892 | 5.2 | 6.5E-22 | nde_ctg_10 | 1.4 | 1.8E-02 | * | ||
| Far upstream element-binding protein 3 | NP_001034426.1 | fl_ctg_614 | −31.2 | 0 | ph_ctg_666 | −1.9 | 1.8E-02 | * | ||
| Fibulin-2 | EKC31789.1 | fl_ctg_604 | −3.9 | 6.4E-03 | ph_ctg_1457 | −3.8 | 5.0E-08 | |||
| Hemicentin-1 | EKC33127.1 | fl_ctg_1013 | 15.8 | 4.9E-21 | ph_ctg_1370 | −2.4 | 0 | * | ||
| Krueppel-like factor 11 | EKC40859.1 | fl_ctg_1120 | 3.9 | 4.1E-07 | ph_ctg_245 | 3.6 | 5.0E-02 | |||
| Outer dense fiber protein 3 | NP_001171751.1 | fl_ctg_846 | −12.2 | 8.1E-04 | ph_ctg_803 | 4.0 | 4.9E-02 | * | ||
| Putative accessory gland protein | ABG01813.1 | fl_ctg_498 | 21.0 | 5.2E-20 | ph_ctg_280 | 2.2 | 4.5E-05 | * | ||
| Tribbles-like protein 2 | EKC22747.1 | fl_ctg_1325 | 10.4 | 2.3E-164 | ph_ctg_2106 | 1.9 | 3.0E-09 | * | ||
| Water and ammonia transporting aquaporin | EKC35418.1 | fl_ctg_1403 | 2.3 | 1.5E-04 | ph_ctg_1774 | −1.6 | 2.4E-05 | * | ||
| Epithelial membrane protein 2 | EKC25041.1 | fl_ctg_113 | 17.2 | 3.2E-07 | ||||||
| Liver stage antigen 3 precursor | XP_002257838.1 | fl_ctg_642 | −19.1 | 1.3E-15 | ||||||
| Semenogelin II precursor | ABO52944.1 | fl_ctg_628 | −16.5 | 0 | ||||||
| Dexras1 | XP_002430253.1 | ph_ctg_1269 | 24.8 | 1.7E-02 | ||||||
Genes were organized in broad functional categories. Absence of contig, fold change, and p-value information for a given gene indicates that no orthologous sequence was detected in that particular species. Italicized contig identifiers indicate that the gene did not fit criteria for significance (≥1.5 fold change, p<0.05) in the particular species. * indicates significantly different fold changes (FC) between species for a given gene based on a t-test of replicated fold change values (p<0.05). Differences in fold changes between species in each category were assessed for significance using a paired t-test (p<0.05) with values given next to the category titles. Additional information for each contig is available in Table S3.