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. 2014 Feb 25;9(2):e89748. doi: 10.1371/journal.pone.0089748

Figure 2. SF-E effects on microglial cell viability and ROS production.

Figure 2

(A) Exposure of SF-E (0 to 80 µg/mL) to BV-2 microglial cells for 16 h did not alter cell viability as assayed by WST1. Data are expressed as the mean ± SEM from 3 individual experiments and analyzed by one-way ANOVA (p = 0.6828). SF-E inhibits LPS+IFNγ-induced ROS production in BV-2 (B) and HAPI (C) microglial cells. SF-E (0 to 80 µg/mL) were applied to cells 1 h prior to exposure to a combination of LPS (100 ng/mL) and IFNγ (10 ng/mL) for 12 h. ROS production was measured using CM-H2DCFDA as described in the text. Results are expressed as the mean ± SEM (n = 3) and analyzed by two-way ANOVA with Bonferroni post-tests. *p<0.05; **p<0.01; ***p<0.001 as compared to the respective LPS+IFNγ-stimulated group.