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. Author manuscript; available in PMC: 2014 Feb 25.
Published in final edited form as: Sci Transl Med. 2013 Sep 25;5(204):204ra130. doi: 10.1126/scitranslmed.3006516

Fig. 2. ICMV vaccination elicits durable CD8+ T cell responses with enhanced functionality.

Fig. 2

C57BL/6 mice were immunized intratracheally with ICMV or soluble OVA vaccines on day 0 and boosted on day 28. (A) Frequencies and absolute numbers of OVA-specific CD8+ T cells in mdLNs, lungs, blood, and spleen were tracked over time by SIINFEKL-MHC tetramer staining. (B and C) On day 35, cells isolated from lungs and spleen were restimulated ex vivo with SIINFEKL peptide, and expression of intracellular interferon-γ (IFN-γ) and/or tumor necrosis factor–α (TNF-α) (B) and granzyme B (C) among CD8+ T cells was determined by flow cytometry. (D) Frequency of long-lived effector memory (CD127hiKLRG1lo) CD8+ T cell precursors in multiple compartments was determined on day 7 after prime and boost by flow cytometry analysis of tetramer+ CD8+ T cells. Data are means ± SEM of two to three independent experiments conducted with n = 3 to 4 animals per group. *P < 0.05, **P < 0.01, ***P < 0.001, by two-way ANOVA (A, B, and D) or one-way ANOVA (C).