Table 1. PCR conditions for the detection of V. vulnificus and V. parahaemolyticus virulence genes.

	Primer (forward & reverse)/
Primer	Probe Concentrations (nM)	PCR conditions
Vibrio vulnificus/vvh	400/240	1x: 95°C for 60 s; 41x: 95°C for 5 s, 59°C for 45 s
Vibrio vulnificus/vcgC	250/180	1x: 95°C for 10 m; 40x: 95°C for 15 s, 60°C for 90 s
Vibrio parahaemolyticus/tlh	200/150	1x: 95°C for 10 m; 45x: 95°C for 5 s, 66°C for 45 s
Vibrio parahaemolyticus/tdh trh	200/75	1x: 95°C for 60 s; 50x: 95°C for 5 s, 59°C for 45 s