Table 1. PCR conditions for the detection of V. vulnificus and V. parahaemolyticus virulence genes.
Primer (forward & reverse)/ | ||
Primer | Probe Concentrations (nM) | PCR conditions |
Vibrio vulnificus/vvh | 400/240 | 1x: 95°C for 60 s; 41x: 95°C for 5 s, 59°C for 45 s |
Vibrio vulnificus/vcgC | 250/180 | 1x: 95°C for 10 m; 40x: 95°C for 15 s, 60°C for 90 s |
Vibrio parahaemolyticus/tlh | 200/150 | 1x: 95°C for 10 m; 45x: 95°C for 5 s, 66°C for 45 s |
Vibrio parahaemolyticus/tdh trh | 200/75 | 1x: 95°C for 60 s; 50x: 95°C for 5 s, 59°C for 45 s |