Figure 5. Increased alkaline tolerance of GsPPCK3-SCMRP overexpression transgenic alfalfa.

a. Semi-quantitative RT-PCR analysis of GsPPCK3 and SCMRP expression levels in WT and trangenic lines. b. Western blot identification of GsPPCK3-SCMRP transgenic alfalfa. c. Growth performance of WT and transgenic lines after alkali treatment. Photographs were taken 15 days after initial treatment. d. Shoot length of the WT and transgenic lines. e. Shoot weight and root weight of the WT and transgenic lines. For phenotypic analysis of GsPPCK3-SCMRP transgenic alfalfa, the 4-week-old plants were treated with 1/8 Hoagland nutrient solution containing either 0, or 100, or 150 mM NaHCO₃ every 3 days for a total of 15 days.