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. 2014 Feb 25;9(2):e89927. doi: 10.1371/journal.pone.0089927

Figure 2. Reduced development of endothelial cellular phenotypes in CACs from HHT patients.

Figure 2

CACs derived from patients with HHT appeared smaller in size and more rounded (a) when compared to the spindle-shaped, elongated morphology of CACs derived from control subjects (b). Dil-Ac-LDL/UEA-1 double positive cells (a representative image shown in c. Orange: double positive; green: UEA-1 positive; red: Dil-Ac-LDL positive; blue is nuclear stain) and VEGFR2 positive cells (a representative image shown in d. Green: VEGFR2 positive; red is nuclear stain) in CACs were analyzed by immunofluorescence microscopy. The results showed that percentage of Dil-Ac-LDL/UEA-1 double positive cells and percentage of VEGFR2 positive cells, both were significantly lower in CACs isolated from HHT patients (e and f). *p<0.05, compared with healthy controls. **p<0.01, compared with healthy controls.