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. 2014 Feb 25;9(2):e89806. doi: 10.1371/journal.pone.0089806

Figure 5. Growth inhibition and apoptosis induction of mouse orthotopic bladder tumors by 2 µM resveratrol intravesical instillation for 13 times in 2 day intervals.

Figure 5

A. Schematic diagram of study design and dosing schedule. Treatments began 3 days after implantation (asterisks). B. Upper: Urethral catheterization of nude mice; Lower: the sizes of a pair of tumor-bearing urinary bladders without and with 2 h 200 µM resveratrol intravesical instillation for 13 times. C. Comparison of tumor burdens between resveratrol instillation group and the untreated control at Day 28 after tumor implantation. Normal nude mice (the same weeks old) bladders free of tumor implantation as control. *, compared with N group or Res group, P<0.01; #, compared with N group, P>0.05. D. H&E staining and TUNEL assay (the insets) for histological and apoptosis evaluation of urinary bladder tissues with intravesical resveratrol treatment (2 h 200 µM resveratrol for 13 times in 2 day intervals). Normal: the bladder of a nude mouse; Control: the bladder bearing orthotopic transplanted tumor without resveratrol treatment (0.9% NaCl treatment); Res: the bladder bearing orthotopic transplanted tumor with 200 µM resveratrol treatment for 2 h and 13 times. E. Evaluation of apoptotic indexes among Normal, Control and Res groups.