Skip to main content
. 2014 Feb 25;9(2):e89824. doi: 10.1371/journal.pone.0089824

Figure 2. Syndecan-1 knockout leads to loss of gene expression of mature vascular smooth muscle cell (vSMC) markers.

Figure 2

Real time PCR analyses of vSMC differentiation-related markers and tissue factor in vSMCs under the following treatments: (A) control (1% FBS), (B) medium containing 30 µg/mL heparin, and (C) medium containing 30 µg/mL heparin and 5 ng/mL TGF-β1. These analyses indicated that vSMCs were more differentiated and expressed higher levels of vSMC-specific differentiation markers in WT vSMCs relative to S1KO vSMCs. In addition, SMemb, the embryonic form of myosin heavy chain, was higher in S1KO vSMCs after treatment with heparin and TGF-β1. *Statistically significant difference with WT group under similar culture conditions (p<0.05).