Figure 7. Anti-SiglecF-680 binds to eosinophils and macrophages.

Fluorescence microscopy of cryosections from lungs of EAAD mice injected with anti-SiglecF-680 (A), confirms the binding of anti-SiglecF-680 (in green) to eosinophils (EMBP-positive, arrows in merge) and more weakly to macrophages, which were counterstained with anti-CD68 (magenta, arrow heads in merge). Lungs from healthy controls injected with anti-SiglecF-680 (B) have a low number of Siglec-F positive cells, which are all CD68-positive and therefore most probably represent macrophages. Nuclei are stained blue with DAPI. Scale bars = 20 µm.