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. 2014 Feb 25;9(2):e90072. doi: 10.1371/journal.pone.0090072

Figure 1. Suppression of the yeast sec12-1 by the plant AtSec12 homologue.

Figure 1

A) Total protein extracts of yeast wild-type (WT) and sec12-1 mutant cells untransformed or transformed with the empty vector (pVV) or expressing plant AtSEC12 were resolved by SDS-PAGE and immunoblotted with anti-AtSec12 antibodies. B) Yeast cell cultures grown to mid-exponential phase at 25°C were spotted on YPD medium containing MATa bar1 mutant cells to determine α-factor secretion at permissive (25°C) and various restrictive temperatures (30°C, 35°C and 37°C). C) Localization of a COPII cargo the SNARE GFP-Snc1 in the sec12-1 mutant transformed with either pVV (empty) or AtSec12 plasmids was determined by epifluorescence observation (with GFP and Differential interference contrast (DIC) filters) on cell cultures grown to mid-exponential phase at 25°C. D) Total protein extracts from wild-type (WT) cells or sec12-1 mutant cells transformed with pVV or AtSEC12 plasmid and with GFP-Snc1 vector and grown at 25°C were resolved by SDS-PAGE and immunoblotted with anti-GFP antibodies.