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Triton-X100 extract of 15 day-old seedlings of Col-0 A. thaliana was immunoprecipitated with anti-AtSar1B serum. The resulting immunoprecipitate was collected with Protein A/Protein G/γ-Bind-Sepharose beads and analyzed by Western blotting using anti-AtSar1B, anti-AtSec12, anti-AtSec13A, anti-AtSec23B serum. A total cell extract was added as control (Input).
