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. 2014 Feb 25;9(2):e90025. doi: 10.1371/journal.pone.0090025

Figure 2. Pyk3 and Phg2 are required for full transcriptional activation of STATc-dependent genes.

Figure 2

Real time PCR analysis of selected genes was carried out with cDNAs from untreated cells and from cells treated for 15 minutes with either 200(A), 20 mM 8-Br-cGMP (B) or 30 µM BHQ (C). The differential expression was investigated in Ax2 wild-type, pyk3−, phg2−, pyk3−/phg2−, and STATc− cells upon treatment with sorbitol and 8-Br-cGMP (A, B) or in Ax2 wild-type, pyk3−, phg2−, and pyk3−/phg2− cells upon treatment with BHQ (C). The data are expressed as means of fold change in comparison to untreated cells. A students t-test was performed and significance values were calculated: p-value <0.05 = */▪; p-value <0.01 = **/▪▪ ; p-value <0.001 = ***/▪▪▪ . Fold changes and standard deviations of six measurements from three independent experiments are shown. *Statistically significant changes, compared with AX2 cells. ▪Statistically significant changes, compared with pyk3− cells. N/A: not applicable.