Table 2. Primer pairs used for quantitative Real-Time PCR analysis.
dictyBase ID | GeneName | Sequence |
DDB_G0293532 | dstc | Forward: 5′-CAATTACTTTGTGGCACTCG-3′Reverse: 5′-CCAAATTTGAGGGTTACTGG-3′ |
DDB_G0282145 | ptpC | Forward: 5′-ACCGATATGGGTATTCGTTC-3′Reverse: 5′-TGTTGTGGTGGGAATTTTAG-3′ |
DDB_G0283385 | pyk1 | Forward: 5′-AAGTTGGCTTTGGATATTGC-3′Reverse: 5′-GCCATGTAGGGAATACAACC-3′ |
DDB_G0285321 | pyk2 | Forward: 5′-CTGTACACACTACTAGTGGTGG-3′Reverse: 5′-CTACCTCTAGGTCTCATATGAG-3′ |
DDB_G0289001 | pyk3 | Forward: 5′-TTTTATGGGTGCCTGTATTG-3′Reverse: 5′-TCATCGCTTAAAGTTGTTGC-3′ |
DDB_G0283699 | phg2 | Forward: 5′-GAGAACCCTGACAAAAGACC-3′Reverse: 5′-GAGGAAGAAGGCACAACAAC-3′ |
DDB_G0282895 | morn | Forward: 5′-ATTGGATCACCACCATTACC-3′Reverse: 5′-TCATCACCACTTGAAGCAAC-3′ |
DDB_G0293416 | abcB1 | Forward: 5′-CAGCTATCAATGTTTCAGGTG-3′Reverse: 5′-CCACGATACCAAACTGGATCC-3′ |
Oligonucleotide primers were designed on the basis of sequence information and purchased from Metabion Corp. (Munich, Germany).